製品の概要

  • 製品名Anti-Peroxiredoxin 6 antibody
    Peroxiredoxin 6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin 6
  • 特異性ab59543 reacts with Peroxiredoxin 6.
  • アプリケーション適用あり: Flow Cyt, WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Rat) Peroxiredoxin 6

  • ポジティブ・コントロール
    • Rat and mouse lung and human brain.
  • 特記事項Staining pattern: cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. Stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/4000. Predicted molecular weight: 25 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.

ターゲット情報

  • 機能Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • 配列類似性Belongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • 細胞内局在Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
    • 24 kDa protein antibody
    • 9430088D19Rik antibody
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    • Acidic calcium-independent phospholipase A2 antibody
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    • Antioxidant protein 2 antibody
    • AOP2 antibody
    • Aop2 rs3 antibody
    • Brp 12 antibody
    • Ciliary body glutathione peroxidase antibody
    • CP 3 antibody
    • EC 1.11.1.15 antibody
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    • EC 3.1.1. antibody
    • Epididymis secretory sperm binding protein Li 128m antibody
    • GPx antibody
    • HEL S 128m antibody
    • KIAA0106 antibody
    • Liver 2D page spot 40 antibody
    • Ltw4 antibody
    • Lvtw 4 antibody
    • MGC46173 antibody
    • mKIAA0106 antibody
    • Non selenium glutathione peroxidase antibody
    • Non-selenium glutathione peroxidase antibody
    • NSGPx antibody
    • ORF06 antibody
    • OTTHUMP00000032693 antibody
    • p29 antibody
    • Peroxiredoxin-6 antibody
    • Peroxiredoxin6 antibody
    • PHGPx antibody
    • Phospholipase A2 lysosomal antibody
    • PLA2 antibody
    • PRDX 6 antibody
    • Prdx5 antibody
    • PRDX6 antibody
    • Prdx6 rs3 antibody
    • PRDX6_HUMAN antibody
    • PRX antibody
    • Red blood cells page spot 12 antibody
    • Thiol specific antioxidant protein antibody
    see all

Anti-Peroxiredoxin 6 antibody 画像

  • Lane 1 : Anti-Peroxiredoxin 6 antibody (ab59543)
    Lane 2 : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle whole tissue lysates
    Lane 2 : Mouse skeletal muscle whole tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • ab59543 at 1/100 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by Immunohistochemistry. Secondary antibody anti-rabbit IgG conjugated to Cy3 (1/100).
  • ab59543 at 1/1000 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by IHC-P. Secondary antibody Donkey anti-rabbit conjugated to biotin.
  • All lanes : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/2500 dilution

    Lane 1 : Wild-type rat cortex
    Lane 2 : Transgenic (APP/PS1) male mouse cortical lysate
    Lane 3 : Transgenic (APP/PS1) male mouse cortical lysate
    Lane 4 : Transgenic (APP/PS1) male mouse cortical lysate
    Lane 5 : Transgenic (APP/PS1) male mouse cortical lysate
    Lane 6 : Wild-type male mouse cortical lysate
    Lane 7 : Wild-type male mouse cortical lysate
    Lane 8 : Wild-type male mouse cortical lysate
    Lane 9 : Wild-type male mouse cortical lysate

    Secondary
    Alexa Fluor 680-conjugated goat anti rabbit IgG polyclonal antibody

    Predicted band size : 25 kDa
    Observed band size : ~25 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr Martin Broadstock

    Western blot showing detection of Peroxiredoxin 6 in mouse and rat cortical lysates by ab59543.

    See Abreview

  • ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab59543 staining Peroxiredoxin 6 in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    See Abreview

Anti-Peroxiredoxin 6 antibody (ab59543) 使用論文

This product has been referenced in:
  • Wang Y  et al. Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis. Cancer Sci 107:250-7 (2016). Rat . Read more (PubMed: 26679080) »
  • Trevisan R  et al. Zinc causes acute impairment of glutathione metabolism followed by coordinated antioxidant defenses amplification in gills of brown mussels Perna perna. Comp Biochem Physiol C Toxicol Pharmacol 159:22-30 (2014). IF ; Other . Read more (PubMed: 24095941) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain tissue)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification Brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 22 2016

Application Western blot
Sample Human Tissue lysate - whole (Brain Tissue)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification Brain Tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HEK 293)
Permeabilization Yes - 0.025%TritonX100
Specification HEK 293
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formalin
Username

Abcam user community

Verified customer

投稿 Feb 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW264.7)
Specification RAW264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount 50 µg
Specification Skeletal muscle (AT)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

投稿 Sep 28 2012

Thank you for your reply.

Blocking reagents also are subject tooptimization.

If your customer needs a strong blocking solution for their experiment, I can confirm that the combination of BSA and serum is optimal.

Glycine i...

Read More

The particular blocking step your customer mentioned is our standard procedure for ICC/IF.
I have attached our extended standard protocol for IHC-P from our website to this email for her convenience.


As for the dilution range, this wi...

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Thank you for your message. It is regrettable this has not worked for your customer and I apologize for the inconvenience.

Before proceeding, I am sorry I am not able to trace the order number provided, or an order from Italy on that date. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"