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Recombinant human fragment protein (without mitochondrial leader sequence) purified from E.coli.
Our Abpromise guarantee covers the use of ab16942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 24 kDa.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab16942 observed at 17 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab16942 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab16942 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung, respiratory epithelium tissue labeling Peroxiredoxin with ab16942.
ab16942 has not yet been referenced specifically in any publications.