Rabbit polyclonal to Peroxiredoxin 4
Mouse, Rat, Cow
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Peroxiredoxin 4.
This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; HEK293; Raji; HepG2.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).
Use a concentration of 5 µg/ml.
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Information by UniProt
Antioxidant enzyme 372 antibody
Antioxidant enzyme AOE372 antibody
AOE37 2 antibody
Western blot - Peroxiredoxin 4 antibody (ab95232)
All lanes :
Anti-Peroxiredoxin 4 antibody (ab95232) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 5 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
27 kDa (
why is the actual band size different from the predicted?
Additional bands at:
20 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
The Peroxiredoxin 4 protein sequence contains a 37 residue signal sequence at the N-terminus. The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4.
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 4 antibody (ab95232)
ICC/IF image of ab95232 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (
, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.
has not yet been referenced specifically in any publications.
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