製品の概要

  • 製品名
    Anti-Peroxiredoxin 4 antibody
    Peroxiredoxin 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin 4
  • 特異性
    This antibody is specific for Peroxiredoxin 4 and shows no cross reactivity with other Peroxiredoxin isoforms.
  • アプリケーション
    適用あり: WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Other Immunogen Type corresponding to Human Peroxiredoxin 4. Purified human recombinant Peroxiredoxin 4 from K562 cells.

  • ポジティブ・コントロール
    • PC3 cell lysate (WB) TSU-Pr1 cells (ICC)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 33 kDa).

Detects a band of approximately 23 kDa and 20 kDa representing Pro-Peroxiredoxin 4 and mature Peroxiredoxin 4 respectively.

ICC 1/500.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
  • 配列類似性
    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 細胞内局在
    Cytoplasm. Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antioxidant enzyme 372 antibody
    • Antioxidant enzyme AOE372 antibody
    • AOE37 2 antibody
    • AOE37-2 antibody
    • AOE372 antibody
    • EC 1.11.1.15 antibody
    • Peroxiredoxin IV antibody
    • Peroxiredoxin-4 antibody
    • Peroxiredoxin4 antibody
    • PRDX 4 antibody
    • Prdx4 antibody
    • PRDX4_HUMAN antibody
    • PRX 4 antibody
    • Prx IV antibody
    • Prx-IV antibody
    • PRX4 antibody
    • PrxIV antibody
    • Thioredoxin dependent peroxide reductase A0372 antibody
    • Thioredoxin Peroxidase (Antioxidant Enzyme) antibody
    • Thioredoxin peroxidase antibody
    • Thioredoxin peroxidase AO372 antibody
    • Thioredoxin-dependent peroxide reductase A0372 antibody
    • TRANK antibody
    see all

画像

  • ab15574 staining Peroxiredoxin 4 in TSU-Pr1 cells.
  • ab15574 staining Peroxiredoxin 4 (green) in Mouse bone marrow by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and incubated with primary antibody (1/100 in 0.05% Saponin/PBS) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Palande K  et al. Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early endosome interface. J Cell Sci 124:3695-705 (2011). Read more (PubMed: 22045733) »

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bonemarrow)
Specification
Bonemarrow
Fixative
Paraformaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Apr 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HELA)
Specification
HELA
Fixative
3% Paraformaldehyde
Permeabilization
No
Blocking step
NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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