製品の概要

  • 製品名Anti-Peroxiredoxin 2 antibody
    Peroxiredoxin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin 2
  • 特異性ab59539 reacts with Peroxiredoxin 2.
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Cynomolgus Monkey, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide:

    VVDGAFKEVKLS

    , corresponding to amino acids 20-31 of Human Peroxiredoxin 2 conjugated to diphtheria toxin.

  • ポジティブ・コントロール
    • Rat lung and kidney tissue.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/4000. Predicted molecular weight: 22 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • 配列類似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Anti-Peroxiredoxin 2 antibody 画像

  • ICC/IF image of ab59539 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39539, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab59539 (1/2000) staining peroxiredoxin 2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of submucosal infiltrating leukocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-Peroxiredoxin 2 antibody (ab59539) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 37 kDa,49 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • All lanes : Anti-Peroxiredoxin 2 antibody (ab59539) at 1/5000 dilution

    Lane 1 : Whole tissue lysate prepared from murine liver
    Lane 2 : Whole tissue lysate prepared from murine liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 conjugated goat anti-rabbit polyclonal at 1/5000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22,45 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-Peroxiredoxin 2 antibody (ab59539) 使用論文

This product has been referenced in:
  • Klebe S  et al. Peroxiredoxin I and II in Human Eyes: Cellular Distribution and Association with Pterygium and DNA Damage. J Histochem Cytochem N/A:N/A (2013). IHC-P, WB ; Human . Read more (PubMed: 24152995) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 50 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 50 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Thank you for your reply.

At the time of your review, we had no direct information regarding whether the product would work in mouse tissues. As such we had listed the product a 'predicted to work' based sequence homology of the immunogen and...

Read More

Thank you for submitting your Abreview of ab59539 in mouse.

At the time of this Abreview we had not as yet tested ab59539. Thank you so much for testing this antibody in mouse.We appreciate very much your efforts and are sorry to hear that the...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount 50 µg
Specification Skeletal muscle (AT)
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

投稿 Sep 28 2012

Thank you for your honest feedback. I agree that the vial of ab59539 you received does not appear to be working as expected. I am very sorry for the trouble. As per our guarantee, if this antibody has been purchased within 6 months of submitting this r...

Read More
Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Loading amount 20 µg
Specification Skeletal Muscle
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Maria Insenser

Verified customer

投稿 Jan 26 2012

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Recombinant Prdx2)
Specification Recombinant Prdx2
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type Indirect
Username

Abcam user community

Verified customer

投稿 Mar 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"