製品の概要

  • 製品名Anti-Peroxiredoxin 2 antibody
    Peroxiredoxin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin 2
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Peroxiredoxin 2 aa 104-115.
    Sequence:

    LLADVTRRLSED

  • ポジティブ・コントロール
    • TSU PR1 cells (ICC) PC3 cell lysate (WB)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
ICC 1/500.

ターゲット情報

  • 機能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • 配列類似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis secretory sperm binding protein Li 2a antibody
    • HEL S 2a antibody
    • MGC4104 antibody
    • Natural killer cell enhancing factor B antibody
    • Natural killer cell-enhancing factor B antibody
    • Natural Killer Enhancing Factor B antibody
    • NKEF B antibody
    • NKEF-B antibody
    • NKEFB antibody
    • Peroxiredoxin-2 antibody
    • PRDX 2 antibody
    • PRDX2 antibody
    • PRDX2_HUMAN antibody
    • PrP antibody
    • PRX2 antibody
    • PRXII antibody
    • PTX1 antibody
    • TDPX1 antibody
    • Thiol Specific Antioxidant 1 antibody
    • Thiol specific antioxidant protein antibody
    • Thiol-specific antioxidant protein antibody
    • Thioredoxin Dependent Peroxide Reductase 1 antibody
    • Thioredoxin peroxidase 1 antibody
    • Thioredoxin-dependent peroxide reductase 1 antibody
    • Torin antibody
    • TPX1 antibody
    • TSA antibody
    see all

Anti-Peroxiredoxin 2 antibody 画像

  • Staining of Peroxiredoxin 2 in TSU PR1 cells using ab15572.
  • ICC/IF image of ab15572 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15572, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab15572 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15572, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Peroxiredoxin 2 antibody (ab15572) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Jurkats)
Specification Jurkats
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

投稿 Jan 10 2014

Based on information from the originator, this antibody is specific for peroxiredoxin 2 and does not show cross-reactivity with other isoforms.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEPG2, HEK293)
Loading amount 10 µg
Specification HEPG2, HEK293
Blocking step BSA and non fat dry milk as blocking agent for 30 minute(s) · Concentration: 3%, 2%
Username

Abcam user community

Verified customer

投稿 Nov 30 2006

Thank you for your enquiry. I have performed an alignment of the immunogen seqeunces used to raise ab15571 and ab15572 with the sequence you have provided me with. It is available at the link below for the next 24 hours. http://www.ebi.ac.uk/cg...

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This antibody does not contain total Ig or buffer because it is antiserum. Since it is antiserum it is also not purified. This antibody cannot be supplied without the sodium azide, my apologies for the inconvenience. Please do not hesitate to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"