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RabMAb

Anti-Peroxiredoxin 1 抗体 [EPR5434] (ab109506)

製品の概要

  • 製品名
    Anti-Peroxiredoxin 1 antibody [EPR5434]
    Peroxiredoxin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5434] to Peroxiredoxin 1
  • 特異性
    Corresponding to residues in Human Peroxiredoxin 1
  • アプリケーション
    適用あり: WB, IP, ICC, ICC/IF, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    ASynthetic peptide corresponding to residues near the C-terminus of Human Peroxiredoxin 1.

  • ポジティブ・コントロール
    • WB: 293T, K562 or U87-MG cell lysate. IHC-P: Human liver or kidney tissue. IF/ICC: HeLa cell line
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
    • 配列類似性
      Belongs to the ahpC/TSA family.
      Contains 1 thioredoxin domain.
    • 翻訳後修飾
      Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
    • 細胞内局在
      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Heme binding 23 kDa protein antibody
      • MSP23 antibody
      • Natural killer cell-enhancing factor A antibody
      • NKEF A antibody
      • NKEF-A antibody
      • NKEFA antibody
      • OSF3 antibody
      • Osteoblast specific factor 3 antibody
      • PAG antibody
      • Paga antibody
      • PAGB antibody
      • Peroxiredoxin-1 antibody
      • PRDX1 antibody
      • PRDX1_HUMAN antibody
      • Proliferation associated gene A antibody
      • Proliferation-associated gene protein antibody
      • PRX1 antibody
      • PrxI antibody
      • TDPX2 antibody
      • Thioredoxin peroxidase 2 antibody
      • Thioredoxin-dependent peroxide reductase 2 antibody
      see all

    Anti-Peroxiredoxin 1 antibody [EPR5434] 画像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109506 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109506 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab109506 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ICC/IF image of ab109506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109506, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Peroxiredoxin 1 with unpurified ab109506 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa

    Anti-Peroxiredoxin 1 antibody [EPR5434] (ab109506) 使用論文

    ab109506 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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