Recombinant full length protein corresponding to Human Peroxiredoxin 1.
Our Abpromise guarantee covers the use of ab70666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | Use at an assay dependent concentration. | |
WB | 1/20000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). | |
IP | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. |
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab70666 observed at 23 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab70666 was shown to specifically react with Peroxiredoxin when Peroxiredoxin knockout samples were used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. ab70666 and ab181602 (loading control to GAPDH) were diluted 1/20 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling Peroxiredoxin 1 with ab70666.
Immunoprecipitation analysis using ab70666.
Lane 1: HeLa cell lysate
Lane 2: Immunoprecipitate from HeLa cell lysate (200 µg) using ab70666 (1 µg)
Lane 3: Immunoprecipitate from HeLa cell lysate (200 µg) using ab70666 (2 µg)
Lane 4: Immunoprecipitate from PBS using ab70666 (2 µg)
ab70666 was also used for western blot at 1/20000 overnight at 4°C.
Incubated with the secondary antibody at 1/5000 for 1 hour at room temperature.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
ab70666 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"