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Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)
Our Abpromise guarantee covers the use of ab79483 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 125 kDa.|
|IHC-P||1/50 - 1/100.|
IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)
ab79483 has not yet been referenced specifically in any publications.