Anti-PERK 抗体 (ab79483)
Key features and details
- Rabbit polyclonal to PERK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PERK antibody
PERK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PERK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)
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ポジティブ・コントロール
- IHC: human colon carcinoma tissue WB: extracts from MCF-7 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 125 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 125 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. -
組織特異性
Ubiquitous. A high level expression is seen in secretory tissues. -
関連疾患
Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain. -
ドメイン
The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. -
翻訳後修飾
Autophosphorylated.
N-glycosylated. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9451 Human
- Entrez Gene: 13666 Mouse
- Entrez Gene: 29702 Rat
- Omim: 604032 Human
- SwissProt: Q9NZJ5 Human
- SwissProt: Q9Z2B5 Mouse
- SwissProt: Q9Z1Z1 Rat
- Unigene: 591589 Human
see all -
別名
- DKFZp781H1925 antibody
- E2AK3_HUMAN antibody
- EC 2.7.11.1 antibody
see all
画像
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All lanes : Anti-PERK antibody (ab79483) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 125 kDa
Observed band size: 125 kDa -
IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, stained with ab79483 at 1/50-1/100 dilution, in the absence and presence of the immunising peptide
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab79483 は 22 報の論文で使用されています。
- Zhang Y et al. Azithromycin alleviates the severity of rheumatoid arthritis by targeting the unfolded protein response component of glucose-regulated protein 78 (GRP78). Br J Pharmacol 179:1201-1219 (2022). PubMed: 34664264
- Li S et al. Sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA2b) mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain. Br J Pharmacol 179:2016-2036 (2022). PubMed: 34811737
- Wang Y et al. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells. Open Med (Wars) 17:1780-1787 (2022). PubMed: 36447525
- Otero A et al. Prion-Associated Neurodegeneration Causes Both Endoplasmic Reticulum Stress and Proteasome Impairment in a Murine Model of Spontaneous Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33466523
- Li X et al. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2a/ATF4/CHOP pathway in breast cancer. Cell Death Dis 12:314 (2021). PubMed: 33762577