製品の概要

  • 製品名Anti-PERK antibody
    PERK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PERK
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N terminal region of human PERK.

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/1000.

ターゲット情報

  • 機能Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
  • 組織特異性Ubiquitous. A high level expression is seen in secretory tissues.
  • 関連疾患Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.
  • 翻訳後修飾Autophosphorylated.
    N-glycosylated.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781H1925 antibody
    • E2AK3_HUMAN antibody
    • EC 2.7.11.1 antibody
    • Eif2ak3 antibody
    • Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
    • Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
    • Heme regulated EIF2 alpha kinase antibody
    • HRI antibody
    • HsPEK antibody
    • Pancreatic eIF2 alpha kinase antibody
    • Pancreatic eIF2-alpha kinase antibody
    • PEK antibody
    • PRKR like endoplasmic reticulum kinase antibody
    • PRKR-like endoplasmic reticulum kinase antibody
    • WRS antibody
    see all

Anti-PERK antibody 画像

  • IHC-P image of PERK staining on human cortical sections using ab77654 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 2 hour at 20°C. ab77654 was diluted 1:500 and incubated with the sections for 18 hours at 4°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)

     

    See Abreview

  • Formalin fixed and paraffin embedded human hepatocarcinoma tissue labelled with ab77654 (at a 1/50 dilution), which was peroxidase conjugated to the secondary antibody, followed by DAB staining.

Anti-PERK antibody (ab77654) 使用論文

ab77654 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Fukomys anselli Tissue sections (Trigeminal ganglion)
Permeabilization No
Specification Trigeminal ganglion
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Dr. Pascal Malkemper

Verified customer

投稿 Apr 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Cortical section)
Specification Cortical section
Permeabilization Yes - Triton-X
Fixative Formaldehyde
Username

Dr. Martin Broadstock

Verified customer

投稿 Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"