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Our Abpromise guarantee covers the use of ab39374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||1/50 - 1/100.|
ab39374 staining Peripherin in Human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/250 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-chicken IgY polyclonal (1/500) was used as the secondary antibody.