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Our Abpromise guarantee covers the use of ab4653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IHC-Fr||Use at an assay dependent concentration. ab4653 is relatively sensitive to aldehyde fixation and so is not recommended for heavily fixed formalin fixed sections of brain.|
Immunohistochemical analysis of rat dorsal root ganglia, staining Peripherin with ab4653.
The DRGs from sham rats were cut to 30 μm and collected free floating in 0.1 m PBS. Sections were blocked with 5% blocking reagent for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) and an Alexa Fluor®594-conjugated donkey anti-mouse IgG (1/400) was used as the secondary antibody.