製品の概要

  • 製品名Anti-Periostin antibody
    Periostin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Periostin
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Periostin .

    (Peptide available as ab90847.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in WB in Human breast tumour tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 0.1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80, 86, 86, 93 kDa (predicted molecular weight: 93 kDa). This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 & 4 of Human Periostin.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • 組織特異性Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • 配列類似性Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • 翻訳後修飾Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fasciclin-I like antibody
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    • OSF-2 antibody
    • OSF2 antibody
    • Osteoblast specific factor 2 (fasciclin I like) antibody
    • Osteoblast specific factor 2 antibody
    • Osteoblast specific factor antibody
    • Osteoblast-specific factor 2 antibody
    • PDLPOSTN antibody
    • Periodontal ligament specific periostin antibody
    • Periostin antibody
    • Periostin isoform thy2 antibody
    • Periostin isoform thy4 antibody
    • Periostin isoform thy6 antibody
    • Periostin isoform thy8 antibody
    • Periostin osteoblast specific factor antibody
    • PN antibody
    • POSTN antibody
    • POSTN_HUMAN antibody
    • RP11 412K4.1 antibody
    see all

Anti-Periostin antibody 画像

  • Anti-Periostin antibody (ab79946) at 1 µg/ml (blocked with 3% milk) + Breast (Human) Cytoplasmic Lysate - tumor tissue (ab30085) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 80,86,87,93 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab79946 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79946, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
  • IHC image of Periostin staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79946, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Periostin antibody (ab79946) 使用論文

This product has been referenced in:
  • Kong P  et al. Lack of specificity of fibroblast-specific protein 1 in cardiac remodeling and fibrosis. Am J Physiol Heart Circ Physiol 305:H1363-72 (2013). IHC ; Mouse . Read more (PubMed: 23997102) »

See 1 Publication for this product

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Thank you for contacting us.

The lot of this antibody that you will receive is 0.9 mg/ml. Please note that another lot in our inventory has a concentration of 0.1 mg/ml. The concentration will vary from one lot to another.

For IH...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
Permeabilization No
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投稿 Oct 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"