製品の概要

  • 製品名
    Anti-Perilipin A+B antibody
    Perilipin A+B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Perilipin A+B
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: MEPVVRRLST QFTAANELAC RGLDHLEEKI PALQYPPEKI ASELKDTIST, corresponding to a region within N terminal amino acids 73-122 of Human Perilipin A+B

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay:1:312500.

ターゲット情報

  • 関連性
    Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
  • 細胞内局在
    Lipid droplet
  • 参照データベース
  • 別名
    • Lipid droplet associated protein antibody
    • PERIA antibody
    • PERIB antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin B antibody
    • PLIN antibody
    see all

画像

  • Anti-Perilipin A+B antibody (ab66514) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab66514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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