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Our Abpromise guarantee covers the use of ab47638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
ab47638 staining Perilipin 3 in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab47638 at 10 µg/ml staining M6RBP1 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).