製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Perilipin 3
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-NQKTPQDAEKDPAK

    , corresponding to internal sequence amino acids 309-322 of Mouse M6PRBP1 (Q9DBG5).

  • ポジティブ・コントロール
    • Mouse Spleen, Mouse Testis, Rat Skin lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
  • 配列類似性
    Belongs to the perilipin family.
  • 細胞内局在
    Cytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 47 kDa mannose 6-phosphate receptor-binding protein antibody
    • 47 kDa MPR binding protein antibody
    • 47 kDa MPR-binding protein antibody
    • Cargo selection protein TIP47 antibody
    • M6PRBP 1 antibody
    • M6PRBP1 antibody
    • Mannose 6 phosphate receptor binding protein 1 antibody
    • Mannose-6-phosphate receptor-binding protein 1 antibody
    • MGC11117 antibody
    • MGC2012 antibody
    • MPR binding protein 47 kDa antibody
    • Perilipin 3 antibody
    • Perilipin-3 antibody
    • Perilipin3 antibody
    • Placental protein 17 antibody
    • PLIN3 antibody
    • PLIN3_HUMAN antibody
    • pp17 antibody
    • Tail interacting protein 47 kD antibody
    • TIP47 antibody
    see all

画像

  • ab118605 at 5 µg/ml staining M6PRBP1 in Mouse Spleen by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • ab118605 at 5 µg/ml staining M6PRBP1 in Mouse Testis by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • Anti-Perilipin 3 antibody (ab118605) at 0.1 µg/ml + Rat Skin lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa

参考文献

This product has been referenced in:
  • Goh VJ  et al. Postnatal Deletion of Fat Storage-inducing Transmembrane Protein 2 (FIT2/FITM2) Causes Lethal Enteropathy. J Biol Chem 290:25686-99 (2015). Read more (PubMed: 26304121) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Jun 07 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse skin)
Specification
mouse skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Feb 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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