Anti-Perilipin-1 抗体 (ab60269)
Key features and details
- Goat polyclonal to Perilipin-1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Perilipin-1 antibody
Perilipin-1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Perilipin-1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Perilipin-1 aa 488-499 (internal sequence).
Sequence:C-PREKPKRRVSDS
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60269の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.01 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
組織特異性
Adipocytes. -
配列類似性
Belongs to the perilipin family. -
翻訳後修飾
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
細胞内局在
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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参照データベース
- Entrez Gene: 520598 Cow
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
see all -
別名
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
画像
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Anti-Perilipin-1 antibody (ab60269) at 0.01 µg/ml + Human adipose lysate (35µg protein in RIPA buffer)
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab60269 は 3 報の論文で使用されています。
- Osorio-Conles Ó et al. Positive Effects of a Mediterranean Diet Supplemented with Almonds on Female Adipose Tissue Biology in Severe Obesity. Nutrients 14:N/A (2022). PubMed: 35807797
- Rodríguez-Castelán J et al. Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits. Biomed Res Int 2017:3795950 (2017). PubMed: 28133606
- Cheung W et al. Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication. J Virol 84:6782-98 (2010). ICC/IF ; Rhesus monkey . PubMed: 20335253