製品の概要

  • 製品名Anti-Pericentrin antibody
    Pericentrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pericentrin
  • アプリケーション適用あり: IP, ICC/IF, ICCmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human Pericentrin (NP_006022.3).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
ICC/IF 1/1000 - 1/5000.
ICC 1/1000 - 1/5000.

Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells.

  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
    • 組織特異性Expressed in all tissues tested, including placenta, liver, kidney and thymus.
    • 関連疾患Microcephalic osteodysplastic primordial dwarfism 2
    • ドメインComposed of a coiled-coil central region flanked by non-helical N- and C-terminals.
    • 細胞内局在Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    Anti-Pericentrin antibody 画像

    • Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
    • Detection of Pericentrin by Western Blot of Immunoprecipitates in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99341 at 3 µg/mg lysate. Anti-Pericentrin antibody ab99342, which recognizes a downstream epitope, was used at 1 µg/ml for subsequent WB detection.

    Anti-Pericentrin antibody (ab99341) 使用論文

    ab99341 has not yet been referenced specifically in any publications.

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