製品の概要

  • 製品名Anti-Pericentrin 1 antibody
    Pericentrin 1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Pericentrin 1
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    ARAIIREGSLEGS

    , corresponding to C terminal amino acids 644-656 of Human Pericentrin1.

  • ポジティブ・コントロール
    • WB: Hela lysate. ICC/IF: T47D cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19841162
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/32000.

ターゲット情報

  • 機能Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.
  • 配列類似性Belongs to the nucleoporin Nup85 family.
  • 細胞内局在Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Nucleus membrane. During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 85 kDa nucleoporin antibody
    • FLJ12549 antibody
    • FROUNT antibody
    • Frount protein antibody
    • Nuclear pore complex protein nucleoporin 85 kDa antibody
    • nuclear pore complex protein nucleoporin Nup75 antibody
    • Nuclear pore complex protein Nup85 antibody
    • Nucleoporin 85 antibody
    • Nucleoporin 85kDa antibody
    • Nucleoporin nucleoporin 85kDa antibody
    • Nucleoporin Nup75 antibody
    • Nucleoporin NUP85 antibody
    • nucleoporin, 75-KD antibody
    • Nucleoporin85 antibody
    • Nup 75 antibody
    • NUP 85 antibody
    • Nup75 antibody
    • nup85 antibody
    • NUP85_HUMAN antibody
    • PCN antibody
    • PCNT 1 antibody
    • PCNT antibody
    • PCNT, formerly antibody
    • PCNT1 antibody
    • Pericentrin antibody
    • Pericentrin, formerly antibody
    • Pericentrin-1 antibody
    • Pericentrin1 antibody
    see all

Anti-Pericentrin 1 antibody 画像

  • ab19044 stained T47D cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA  / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19044 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.



  • Predicted band size : 75 kDa

    ab19044 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer) approx. 75kDa.  Primary incubation was 1 hour.  Detected by chemiluminescence.

    ab19044 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer) approx. 75kDa. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Pericentrin 1 antibody (ab19044) 使用論文

This product has been referenced in:
  • Toda E  et al. FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration. J Immunol 183:6387-94 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19841162) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Paraformaldehyde
Permeabilization Yes - saponin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Derek Passer

Verified customer

投稿 Aug 13 2012

DISCOUNT CODE: ***
Expiration date: November 19, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab19044 in IHC-Fr with mouse tissue, and include this c...

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Thank you for contacting us and for alerting us to the problem you are experiencing with our mouse monoclonal pericentrin antibody ab28144. We take product complaints very seriously, and investigate every product that we feel may not be performing corr...

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Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab19044 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab19044 in ICC/IF. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but please bear i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"