Anti-Perforin 抗体 [CE2.10] (ab16079)

製品の概要

  • 製品名Anti-Perforin antibody [CE2.10]
    Perforin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CE2.10] to Perforin
  • 特異性The antibody does not detect human perforin by Western blot.
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.

  • エピトープRecognizes an epitope in the region amino acids 402-534 of human and mouse perforin.
  • ポジティブ・コントロール
    • Mouse T cell clone B6.1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)This antibody is produced in the medium called optimem with no serum, therefore it does not have to be purified.
  • ポリ/モノモノクローナル
  • クローン名CE2.10
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
WB
  • 追加情報IP: Use at an assay dependent dilution on human and mouse samples.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) from the mouse T cell clone B6.1.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
    • 関連疾患Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 C2 domain.
      Contains 1 EGF-like domain.
      Contains 1 MACPF domain.
    • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
    • 翻訳後修飾N-glycosylated.
    • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cytolysin antibody
      • FLH2 antibody
      • HPLH2 antibody
      • Lymphocyte pore-forming protein antibody
      • P1 antibody
      • PERF_HUMAN antibody
      • perforin 1 (pore forming protein) antibody
      • Perforin 1 antibody
      • Perforin-1 antibody
      • PFP antibody
      • PGFL antibody
      • PIGF antibody
      • PIGF-2 antibody
      • PLGF antibody
      • Pore forming protein antibody
      • prf1 antibody
      • SHGC-10760 antibody
      see all

    Anti-Perforin antibody [CE2.10] (ab16079) 使用論文

    ab16079 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. You are correct, this antibody does not detect human perforin by Western blot. I do apologize as I did not see that statement at first on the online datasheet, and I have updated the datasheet to make it more obvious. I wou...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"