製品の概要

  • 製品名Anti-Perforin antibody [B-D48]
    Perforin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-D48] to Perforin
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human perforin.

  • ポジティブ・コントロール
    • IHC-P: Human spleen FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS
    Sterile-filtered through 0.22 µm and treated to remove endotoxins.
  • Concentration information loading...
  • 精製度Ion Exchange Chromatography
  • ポリ/モノモノクローナル
  • クローン名B-D48
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 関連疾患Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Anti-Perforin antibody [B-D48] 画像

  • IHC image of Perforin staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47225, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Perforin antibody [B-D48] (ab47225) 使用論文

This product has been referenced in:
  • Liu S  et al. Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cells. Cell Death Differ : (2012). Read more (PubMed: 23154388) »
  • Trotta R  et al. The PP2A inhibitor SET regulates granzyme B expression in human natural killer cells. Blood 117:2378-84 (2011). WB ; Human . Read more (PubMed: 21156847) »

See all 3 Publications for this product

Product Wall

You are welcome.

We do not have any data for cross-reactivity with non-primate samples. I tried finding an amino acid sequence for non-primate perforin to align with human perforin, which was the immunogen for ab47225.

The unrevie...

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Thank you for sending the details of your protocol.

I think for PFA-fixed, paraffin-embedded tissue, your protocol is correct. However, our source for this clone has told us that it was only tested in the past for IHC on frozen tissue by col...

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Thank you for bringing this to our attention. To our knowledge, no antigen retrieval should be necessary for histochemistry on frozen tissue sections, or permeabilization assuming your sections are no thicker than 10 microns.

Can you please ...

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Thank you for contacting us. I have doublechecked with the lab and they have confirmed that the antibody ab47225 is provided just in PBS, without any carrier protein or preservative. Format: Phosphate-buffered saline. Sterile-filtered th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"