製品の概要

  • 製品名Anti-Perforin antibody
    Perforin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Perforin
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Rat).

  • ポジティブ・コントロール
    • ICC/IF: SW480 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
  • 追加情報WB: 1/1000. MW ~70kD

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
    • 関連疾患Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 C2 domain.
      Contains 1 EGF-like domain.
      Contains 1 MACPF domain.
    • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
    • 翻訳後修飾N-glycosylated.
    • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cytolysin antibody
      • FLH2 antibody
      • HPLH2 antibody
      • Lymphocyte pore-forming protein antibody
      • P1 antibody
      • PERF_HUMAN antibody
      • perforin 1 (pore forming protein) antibody
      • Perforin 1 antibody
      • Perforin-1 antibody
      • PFP antibody
      • PGFL antibody
      • PIGF antibody
      • PIGF-2 antibody
      • PLGF antibody
      • Pore forming protein antibody
      • prf1 antibody
      • SHGC-10760 antibody
      see all

    Anti-Perforin antibody 画像

    • ICC/IF image of ab7203 stained SW480 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7203, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



    • Predicted band size : 70 kDa
      Western blot detection of rat Perforin from kidney on Day 3 in a glomerular nephritis model.

    Anti-Perforin antibody (ab7203) 使用論文

    This product has been referenced in:
    • Kish DD  et al. Neutrophil expression of Fas ligand and perforin directs effector CD8 T cell infiltration into antigen-challenged skin. J Immunol 189:2191-202 (2012). WB . Read more (PubMed: 22815291) »
    • Mostböck S  et al. Enhanced levels of costimulation lead to reduced effector/memory CD8+ T cell functionality. J Immunol 179:3524-34 (2007). WB ; Mouse . Read more (PubMed: 17785786) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us and your patience, as we were closed over Easter.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may ...

    Read More

    We passed your enquiry on to the originator of this antibody and they have replied with the following response. If you have any further questions, please let us know. Many rat kidney models are known to contain anti-GBM antibody, which has approximatel...

    Read More

    Thank you for your enquiry and yiur interest in our products. Abcam must draw your attention to the disclaimer as set out in its Terms and Conditions: " FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE", and it cannot en...

    Read More

    Not to the best of our knowledge.

    The anti-rat perforin has not been tested for use in flow cytometry. Cross reactivity with other species has also not been determened.

    This antibody has not been tested for use in flow cytometry.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"