Anti-Perforin 抗体 [5B10], prediluted (ab75573)

製品の概要

  • 製品名Anti-Perforin antibody [5B10], prediluted
    Perforin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5B10] to Perforin, prediluted
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to the C terminal region of human Perforin.

  • ポジティブ・コントロール
    • Lymphoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 関連疾患Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Anti-Perforin antibody [5B10], prediluted 画像

  • Formalin-fixed, paraffin-embedded human lymphoma stained with neat ab75573, using peroxidase-conjugate and AEC chromogen. Note granular cytoplasmic staining of lymphocytes.
  • Immunohistochemical analysis of Human skin tissue, staining Perforin with ab75573.

    Tissue was fixed with paraformaldehyde and permeabilized with Tween-20; antigen retrieval was by heat mediation in Tris-EDTA buffer. Samples were incubated with primary antibody (undiluted) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

Anti-Perforin antibody [5B10], prediluted (ab75573) 使用論文

ab75573 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA - Buffer pH 9,0
Permeabilization Yes - Wash-Buffer with Tween from Dako
Username

Mr. Rudolf Jung

Verified customer

投稿 Sep 28 2012

Thank you for your patience whilst I contacted the lab for more information regarding ab75573.

The Perforin datasheet indicates that the antibody was raised to a recombinant prokaryotic protein corresponding to an external region of the C-term...

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Thank you for contacting Abcam.

I have inquired about the Anti-Perforin antibody [5B10] but as it is manufactured off site, I will need to do some more searching to find the necessary information and also to determine if we do know which amino...

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Thank you for contacting us.


As discussed over the phone, anti-Perforin antibody (ab75573) is a preparation of tissue culture supernatant, and as such the exact concentration cannot be accurately determined. It has however been estimate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"