製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Peptide YY
  • 特異性
    Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining.
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Fr, IHC-P, IHC-Rmore details
  • 種交差性
    交差種: Mouse, Rat, Pig
    交差が予測される動物種: Rabbit, Dog, Human
  • 免疫原

    Full length native protein (purified) corresponding to Pig Peptide YY aa 1-36.
    Sequence:

    YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY


    Database link: P68005

  • ポジティブ・コントロール
    • Mammalian colonic endocrine (L/EG) cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 11 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.
IHC-P Use at an assay dependent concentration.
IHC-R 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.

ターゲット情報

画像

  • IHC image of Peptide YY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.
  • ab22663 staining mouse small intestine cells by ICC/IF.  The cells were fixed in acetone, permeabilized in 0.1% TX-100 in AB-solution, and blocked with 10% serum for 30 minutes at 25°C.  The primary antibody was diluted 1/400 in 10% normal goat serum in PBS, 0.1%TX100 and incubated for 24 hours at 4°C.  A FITC conjugated goat anti-rabbit was used as the secondary.

    Blue - DAPI
    Green - Peptide YY

    See Abreview

  • ab22663 staining Peptide YY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue (left, WT; right, Peptide YY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).

参考文献

This product has been referenced in:
  • Brooks L  et al. Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety. Mol Metab 6:48-60 (2017). Read more (PubMed: 28123937) »
  • Aranias T  et al. Lipid-rich diet enhances L-cell density in obese subjects and in mice through improved L-cell differentiation. J Nutr Sci 4:e22 (2015). IHC-P ; Mouse . Read more (PubMed: 26157580) »

See all 6 Publications for this product

レビューと Q&A

Thank you for your reply. After following up with the lab, unfortunately we will not be able to add the immunogenic peptide for ab22663 to our catalog. It is possible that you may be able to have the peptide custom synthesized from an outside company u...

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DISCOUNT CODE: ***
Expiration date: August 24th, 2012

I am very pleased to hear you would like to accept our offer and test ab22663 in human. This code will give you: 1 free primary antibody before the expiration date. To redee...

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I am sorry this product did not contain the expected volume and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22663. To check the status of the order please contact our Customer Servic...

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Thank you for your enquiry. As we discussed, please forward along a representative image of your IHC staining, along with brief descriptions of sample preparation and protocols used. Thanks in advance for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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