Rabbit polyclonal to Pepsinogen II
Rat, Rabbit, Baboon, Macaque monkey, Common marmoset, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Pepsinogen II.
This antibody gave a positive signal in Human Stomach Tumour Tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).
Hydrolyzes a variety of proteins.
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
Information by UniProt
Western blot - Pepsinogen II antibody (ab104595)
Anti-Pepsinogen II antibody (ab104595) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (
) at 10 µg
Human stomach tumor tissue lysate (adenocarcinoma) - total protein (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
40 kDa (
why is the actual band size different from the predicted?
Pepsinogen II has a predicted molecular weight of 42-kDa. The first 16 amino acids of the Pepsinogen II sequence act as a signal sequence. This may explain the migration of the protein at a lower MW than expected.
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