Anti-Pepsinogen I 抗体 [7G3] (ab50123)

製品の概要

  • 製品名
    Anti-Pepsinogen I antibody [7G3]
    Pepsinogen I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7G3] to Pepsinogen I
  • アプリケーション
    適用あり: IHC-P, ELISA, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.15M Sodium chloride, 15mM PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    7G3
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 1 - 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
  • 配列類似性
    Belongs to the peptidase A1 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PEPA3_HUMAN antibody
    • Pepsin A-3 antibody
    • Pepsinogen 3 group I antibody
    • PEPSINOGEN I--SECOND LOCUS antibody
    • Pepsinogen-3 antibody
    • PGA3 antibody
    see all

Anti-Pepsinogen I antibody [7G3] 画像

  • IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

Anti-Pepsinogen I antibody [7G3] (ab50123) 使用論文

ab50123 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Intestine
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 03 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Aug 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2012

Thank you for your enquiry. I am sorry it has taken some time to confirm this information. I can confirm that for sandwich ELISA the following have been used for testing: Capture antibody : ab50607 Anti-Pepsinogen I antibody 2F5 Detection antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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