Anti-Pentraxin 3 抗体 [EPR6699] (ab125007)

製品の概要

  • 製品名Anti-Pentraxin 3 antibody [EPR6699]
    Pentraxin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6699] to Pentraxin 3
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • HL-60 treated with Dexamethasone, HL-60, Human spleen, NIH/3T3, and HUVEC lysates; HUVEC cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
    • 配列類似性Contains 1 pentaxin domain.
    • 翻訳後修飾Glycosylated.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    Anti-Pentraxin 3 antibody [EPR6699] 画像

    • Overlay histogram showing HUVEC cells stained with ab125007 (red line) at 1/40 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions.

    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + NIH/3T3 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 42 kDa
    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + HUVEC cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 42 kDa
    • All lanes : Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/1000 dilution (unpurified)

      Lane 1 : HL-60 treated with Dexamethasone lysate
      Lane 2 : HL-60 lysate
      Lane 3 : Human spleen lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : HUVEC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • Flow cytometric analysis of permeabilized HUVEC cells using ab125007, unpurified, at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Pentraxin 3 antibody [EPR6699] (ab125007) 使用論文

    ab125007 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (cardiac tissue)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 30 µg
    Treatment LPS treated mice
    Specification cardiac tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    投稿 May 07 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"