製品の概要

  • 製品名Anti-PELP1 antibody
    PELP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PELP1
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human MNAR.

    (Peptide available as ab38389.)

  • ポジティブ・コントロール
    • ab32912 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

ターゲット情報

  • 機能Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.
  • 組織特異性Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed.
  • ドメインThe Glu-rich region mediates histones interaction.
    The Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are required for the association with nuclear receptor ESR1.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Cytoplasm. Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors.
  • Information by UniProt
  • 参照データベース
  • 別名
    • glutamic acid- and leucine-rich protein 1 antibody
    • HMX3 antibody
    • MNAR antibody
    • Modulator of non-genomic activity of estrogen receptor antibody
    • Modulator of nongenomic activity of estrogen receptor antibody
    • P160 antibody
    • Pelp1 antibody
    • PELP1 proline glutamic acid leucine rich protein 1 antibody
    • PELP1_HUMAN antibody
    • proline and glutamic acid rich nuclear protein antibody
    • Proline glutamate and leucine rich protein 1 antibody
    • Proline- antibody
    • proline-, glutamic acid- and leucine-rich protein 1 antibody
    • Transcription factor HMX3 antibody
    see all

Anti-PELP1 antibody 画像

  • All lanes : Anti-PELP1 antibody (ab32912) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab32912 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab32912, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of ab32912 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32912, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PELP1 antibody (ab32912) 使用論文

This product has been referenced in:
  • Wang J  et al. Temporal Expression of Pelp1 during Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells. PLoS One 8:e75477 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 24146754) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"