製品の概要

  • 製品名Anti-Pellino 1 antibody
    Pellino 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pellino 1
  • アプリケーション適用あり: ICC, ICC/IF, Flow Cyt, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    SQSNSDTQSV QSTIS

    conjugated to KLH, corresponding to amino acids 121-135 of Human Pellino 1

  • ポジティブ・コントロール
    • Human Ramos cell lysate, mouse Raw cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.

ターゲット情報

Anti-Pellino 1 antibody 画像

  • ab13812 staining Pellino 1 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody..

    See Abreview

  • All lanes : Anti-Pellino 1 antibody (ab13812) at 1/1000 dilution

    Lane 1 : Lysates prepared from human Ramos cell line
    Lane 2 : Lysates prepared from mouse Raw cell line


    Predicted band size : 46 kDa
    Western blot analysis of Pellino1 in human Ramos cell lysate (A) and mouse Raw cell lysate (B) using ab13812 at 1/1000

Anti-Pellino 1 antibody (ab13812) 使用論文

This product has been referenced in:
  • Ma X  et al. Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway. Carcinogenesis 34:1216-23 (2013). Mouse . Read more (PubMed: 23385064) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW264.7)
Specification RAW264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Abcam user community

Verified customer

投稿 Dec 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"