製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PEDF
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PEDF.

    (Peptide available as ab74438.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Tissue lysates: Plasma Total Protein Lysate, Kidney, Ovary (data not shown), Skeletal Muscle (data not shown).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能
    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • 組織特異性
    Retinal pigment epithelial cells and blood plasma.
  • 配列類似性
    Belongs to the serpin family.
  • 発生段階
    Expressed in quiescent cells.
  • ドメイン
    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • 翻訳後修飾
    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在
    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all

Anti-PEDF antibody 画像

  • All lanes : Anti-PEDF antibody (ab74439) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

Anti-PEDF antibody (ab74439) 使用論文

ab74439 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab74439.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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