Anti-PEDF 抗体 (ab180711)
Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PEDF antibody
PEDF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PEDF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow -
免疫原
Recombinant full length protein corresponding to Human PEDF aa 1-200.
Sequence:QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVR SSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIH GTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVL TGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVT KFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLT GSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKL SYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGA GTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP
Database link: P36955 -
ポジティブ・コントロール
- WB: A375 cell lysate and mouse kidney tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180711の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 46 kDa.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 46 kDa. |
ターゲット情報
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機能
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
組織特異性
Retinal pigment epithelial cells and blood plasma. -
配列類似性
Belongs to the serpin family. -
発生段階
Expressed in quiescent cells. -
ドメイン
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
翻訳後修飾
The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 281386 Cow
- Entrez Gene: 5176 Human
- Entrez Gene: 20317 Mouse
- Omim: 172860 Human
- SwissProt: Q95121 Cow
- SwissProt: P36955 Human
- SwissProt: P97298 Mouse
- Unigene: 532768 Human
see all -
別名
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
画像
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All lanes : Anti-PEDF antibody (ab180711) at 1/3000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 60 secondsBlocking buffer: 3% nonfat dry milk in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab180711 は 14 報の論文で使用されています。
- Yan M et al. Transcription factor Foxp1 is essential for the induction of choroidal neovascularization. Eye Vis (Lond) 9:10 (2022). PubMed: 35248156
- Yeh SI et al. Pigment Epithelium-Derived Factor Peptide Promotes Corneal Nerve Regeneration: An In Vivo and In Vitro Study. Invest Ophthalmol Vis Sci 62:23 (2021). PubMed: 33481984
- Yang X et al. CSF1R blockade induces macrophage ablation and results in mouse choroidal vascular atrophy and RPE disorganization. Elife 9:N/A (2020). PubMed: 32234210
- Guo X et al. Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA.hy926 cells. Environ Res 186:109506 (2020). PubMed: 32315827
- McCormick R et al. Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration. Front Bioeng Biotechnol 8:456 (2020). PubMed: 32500067