Anti-PEAR1 抗体 (ab122261)
Key features and details
- Rabbit polyclonal to PEAR1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PEAR1 antibody -
製品の詳細
Rabbit polyclonal to PEAR1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: PSDPNTCSFW ESFTTTTKES HSRPFSLLPS EPCERPWEGP HTCPQPTVVY RTVYRQVVKT DHRQRLQCCH GFYES, corresponding to amino acids 23-97 of Human.
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ポジティブ・コントロール
- IHC: Human bone marrow tissue. ICC/IF: Human cell line U-2 OS.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122261の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. -
組織特異性
Expressed in umbilical vein endothelial cells and platelets (at protein level). Expressed in heart, kidney, skeletal muscle, pancreas, ovary, breast, lung, brain cortex, hypothalamus, spinal cord, dorsal root ganglion, endothelial cells of umbilical cord artery and vein, megakaryocytes, osteoblasts, coronary muscle and erythroid cells. Weakly expressed in peripheral blood leukocytes and macrophages. -
配列類似性
Belongs to the MEGF family.
Contains 9 EGF-like domains.
Contains 1 EMI domain. -
翻訳後修飾
Phosphorylated in the intracellular domain on tyrosine residues (By similarity). Phosphorylated on tyrosine residues by SRC. Tyrosine phosphorylation is detected upon platelet aggregation stimulated by collagen, TRAP and thrombin and platelet-platelet contacts but not after platelet activation. Tyrosine phosphorylation enhanced its association with SHC1 and SHC2. -
細胞内局在
Cell membrane. Detected on the cell surface in resting platelets. - Information by UniProt
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参照データベース
- Entrez Gene: 375033 Human
- Omim: 610278 Human
- SwissProt: Q5VY43 Human
- Unigene: 142003 Human
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別名
- hPEAR1 antibody
- JEDI antibody
- MEGF12 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122261 は論文での使用が確認できていません。