Anti-Pea3 抗体 (ab98068)
Key features and details
- Rabbit polyclonal to Pea3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Pea3 antibody
Pea3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pea3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Pea3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab140322) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: MCF7; K562; Jurkat; HepG2.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab98068の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 54 kDa).
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
-
機能
Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻訳後修飾
Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 2118 Human
- Entrez Gene: 18612 Mouse
- Entrez Gene: 360635 Rat
- Omim: 600711 Human
- SwissProt: P43268 Human
- SwissProt: P28322 Mouse
- Unigene: 434059 Human
- Unigene: 5025 Mouse
-
別名
- Adenovirus E1A enhancer binding protein antibody
- Adenovirus E1A enhancer-binding protein antibody
- E1A F antibody
see all
画像
-
All lanes : Anti-Pea3 antibody (ab98068) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 8 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 33 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
The predicted molecular weight of Pea3 is 54 kDa (SwissProt), however we expect to observe a banding pattern around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab98068 は論文での使用が確認できていません。