製品の概要

  • 製品名Anti-PDXK.1 antibody [OTI5H5]
    PDXK.1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OTI5H5] to PDXK.1
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human PDXK.1 produced in HEK293 cells (NP_003672.1).

  • ポジティブ・コントロール
    • WB: HepG2, HeLa, SVT2, A%49, COS7, Jurkat and MCF7 cell lysates. IHC-P: Human ovary adenocarcinoma, pancreas, endometrium, endometrium adenocarcinoma, bladder carcinoma and lymph nodes. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY PDXK. Flow Cyt: Transfected HEK293T cells.
  • 特記事項

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    The clone number has been updated from 5H5 to OTI5H5, both clone numbers name the same clone.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 35 kDa.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
  • 組織特異性Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa.
  • 配列類似性Belongs to the pyridoxine kinase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • pdxK antibody
    • PDXK_HUMAN antibody
    • PKH antibody
    • PNK antibody
    • Pyridoxal kinase antibody
    • Pyridoxamine kinase antibody
    • Pyridoxine kinase antibody
    • Pyridoxine kinase; antibody
    • Vitamin B6 kinase antibody
    see all

Anti-PDXK.1 antibody [OTI5H5] 画像

  • All lanes : Anti-PDXK.1 antibody [OTI5H5] (ab119051) at 1/200 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 35 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adenocarcinoma of human ovary tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adenocarcinoma of human endometrium tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of carcinoma of human bladder tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • ab119051 at 1/100 dilution staining PDXK.1 in COS7 cells transiently transfected with pCMV6-ENTRY PDXK.1 by Immunofluorescence.
  • ab119051 at 1/100 dilution staining PDXK.1 in HEK293T cells transfected with either pCMV6-ENTRY PDXK.1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by flow cytometry.
  • All lanes : Anti-PDXK.1 antibody [OTI5H5] (ab119051) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PDXK.1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 35 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200975)

Anti-PDXK.1 antibody [OTI5H5] (ab119051) 使用論文

ab119051 has not yet been referenced specifically in any publications.

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