The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling PDS5B with ab70299 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PDS5B antibody (ab70299)
All lanes : Anti-PDS5B antibody (ab70299) at 0.04 µg/ml
Lane 1 : Jurkat cell lysate at 50 µg Lane 2 : Jurkat cell lysate at 15 µg Lane 3 : Jurkat cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Lane 5 : NIH3T3 cell lysate at 50 µg Lane 6 : 293T cell lysate at 50 µg Lane 7 : NIH3T3 cell lysate at 50 µg
Western Blot showing ab70299 at 1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using
Lane 1: ab70298 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane).
Lane 2: ab70299 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane).
Lane 3: control IgG was used for immunoprecipitation.
Detection: Chemiluminescence with exposure time of 10