The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and prostate carcinoma (right) tissues labelling PDS5B with ab70298 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
Western blot - PDS5B antibody (ab70298)
All lanes : Anti-PDS5B antibody (ab70298) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg