製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PDS5B
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1200 and 1250 of human PDS5B

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab70298 was affinity purified using an epitope specific to PDS5B immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • AI646570 antibody
    • Androgen induced proliferation inhibitor antibody
    • Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
    • APRIN antibody
    • AS3 antibody
    • AW212954 antibody
    • CG008 antibody
    • FLJ23236 antibody
    • KIAA0979 antibody
    • mKIAA0979 antibody
    • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) antibody
    • Pds5b antibody
    • RP1 267P19.1 antibody
    • Sister chromatid cohesion protein PDS5 homolog B antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and prostate carcinoma (right) tissues labelling PDS5B with ab70298 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
  • All lanes : Anti-PDS5B antibody (ab70298) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 165 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Western Blot showing ab70298 at 0.1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using ab70298 at 3µg/mg lysate (1 mg/IP loaded/lane).

参考文献

ab70298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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