製品の概要

  • 製品名Anti-PDPK1 (phospho S241) antibody [EPR336(2)]
    PDPK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR336(2)] to PDPK1 (phospho S241)
  • 特異性ab109460 only detects PDPK1 phosphorylated at Serine 241.
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 241 of Human PDPK1.

  • ポジティブ・コントロール
    • 293T cell lysate; Human breast carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-PDPK1 (phospho S241) antibody (Biotin) [EPR336(2)] (ab199740)

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • 組織特異性Appears to be expressed ubiquitously.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • 細胞内局在Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Anti-PDPK1 (phospho S241) antibody [EPR336(2)] 画像

    • All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab109460, at a 1/100 dilution, staining PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109460.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"