製品の概要

  • 製品名
    Anti-PDPK1 antibody - N-terminal
    PDPK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDPK1 - N-terminal
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human PDPK1 conjugated to KLH.

  • ポジティブ・コントロール
    • HL-60 cell lysate. Human hepatocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

ターゲット情報

  • 機能
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • 組織特異性
    Appears to be expressed ubiquitously.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • 細胞内局在
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

画像

  • Anti-PDPK1 antibody - N-terminal (ab71757) at 1/100 dilution + HL-60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ab71757 at 1/50 dilution, staining PDPK1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab71757 has not yet been referenced specifically in any publications.

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