Anti-PDLIM1/CLP36 抗体 [EPR7186(B)] (ab129015)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PDLIM1/CLP36 antibody [EPR7186(B)]
PDLIM1/CLP36 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human heart, L6, 293T, Caco 2, Saos 2, C2C12, Rat lung and Mouse lung lysates; Saos 2 cells. ICC/IF: 293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 9.54 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7186(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129015の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
|
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ICC/IF |
1/100.
|
特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
ICC/IF
1/100. |
ターゲット情報
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機能
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. -
組織特異性
Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 9124 Human
- Entrez Gene: 54132 Mouse
- Entrez Gene: 54133 Rat
- Omim: 605900 Human
- SwissProt: O00151 Human
- SwissProt: O70400 Mouse
- SwissProt: Q3TZ17 Mouse
- SwissProt: P52944 Rat
see all -
別名
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
see all
画像
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All lanes : Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution
Lane 1 : Human heart cell lysate
Lane 2 : L6 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco 2 cell lysate
Lane 5 : Saos 2 cell lysate
Lane 6 : C2C12 cell lysate
Lane 7 : Rat lung cell lysate
Lane 8 : Mouse lung cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 36 kDa -
Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by intracellular flow cytometry or a Rabbit IgG negative control (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab129015 は 2 報の論文で使用されています。
- Lu Y et al. CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1. Theranostics 12:5051-5068 (2022). PubMed: 35836803
- Ono R et al. PDLIM1 inhibits NF-?B-mediated inflammatory signaling by sequestering the p65 subunit of NF-?B in the cytoplasm. Sci Rep 5:18327 (2015). PubMed: 26679095