製品の概要

  • 製品名Anti-PDK2 antibody [EPR1948Y]
    PDK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1948Y] to PDK2
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human PDK2.

  • ポジティブ・コントロール
    • Human heart tissue lysate, mouse heart tissue lysate, rat tissue lysate and human muscle tissue sections.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/30.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 組織特異性Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
  • 配列類似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
    • mitochondrial antibody
    • PDHK2 antibody
    • PDK2 antibody
    • PDK2_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 2 antibody
    • Pyruvate dehydrogenase kinase, isozyme 2 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
    see all

Anti-PDK2 antibody [EPR1948Y] 画像



  • Predicted band size : 46 kDa

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
    Lane 3: SHSY5Y cell lysate (40 ug)
    Lanes 1 - 3: Merged signal (red and green). Green - ab68164 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68164 was shown to specifically react with PDK2 when PDK2 knockout samples were used. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE.  Ab68164 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) ab216773 and IRDye® 680 Goat anti-Mouse IgG (H + L) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/2000 dilution

    Lane 1 : human heart tissue lysate
    Lane 2 : mouse heart tissue lysate
    Lane 3 : rat tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa

    Suggested secondary antibody - goat anti-rabbit HRP (ab6721)

  • ab68164 at 1/100-1/250 dilution staining PDK2 in human muscle tissue sections.

Anti-PDK2 antibody [EPR1948Y] (ab68164) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS One 11:e0150454 (2016). Mouse . Read more (PubMed: 26930489) »
  • Huang JV  et al. Impaired Contractile Recovery after Low-Flow Myocardial Ischemia in a Porcine Model of Metabolic Syndrome. Am J Physiol Heart Circ Physiol : (2013). WB ; Pig . Read more (PubMed: 23335793) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (head and neck cancer)
Permeabilization Yes - triton x 100
Specification head and neck cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative Formaldehyde
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投稿 May 06 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10)
Sample Human Purified protein (head and neck cancer (AMC HN9))
Specification head and neck cancer (AMC HN9)
Treatment 0 5 15um DCA for 24h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"