製品の概要

  • 製品名Anti-PDIA6 antibody
    PDIA6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDIA6
  • アプリケーション適用あり: ICC/IF, IP, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human, Pig
    交差が予測される動物種: Rat, Hamster, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human PDIA6 aa 476-492.
    Sequence:

    DIDLSDVELDDLGKDEL


    (Peptide available as ab11432)

  • ポジティブ・コントロール
    • Human fibrosarcoma HT 1080 whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab11432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).

ターゲット情報

  • 機能May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
  • 組織特異性Expressed in platelets (at protein level).
  • 配列類似性Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 細胞内局在Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Endoplasmic reticulum protein 5 antibody
    • ER protein 5 antibody
    • ERp5 antibody
    • P5 antibody
    • Pdia6 antibody
    • PDIA6_HUMAN antibody
    • Protein disulfide isomerase A6 antibody
    • Protein disulfide isomerase associated 6 antibody
    • Protein disulfide isomerase family A member 6 antibody
    • Protein disulfide isomerase P5 antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A6 antibody
    • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
    • Thioredoxin domain containing protein 7 antibody
    • Thioredoxin domain-containing protein 7 antibody
    • TXNDC7 antibody
    see all

Anti-PDIA6 antibody 画像

  • PDIA6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PDIA6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11432.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 48kDa; PDIA6

  • Western blot of PDIA6 in HT 1080 cell lysate using ab11432. 

  • ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PDIA6 antibody (ab11432) 使用論文

This product has been referenced in:
  • Groenendyk J  et al. Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54 (2014). WB . Read more (PubMed: 24917591) »
  • Wang X  et al. An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic target to inhibit shedding. Biochem Biophys Res Commun 387:476-81 (2009). WB ; Human . Read more (PubMed: 19615970) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab11432.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"