製品の概要

  • 製品名Anti-PDI1 antibody [38H8]
  • 製品の詳細
    Mouse monoclonal [38H8] to PDI1
  • 特異性Determined by screening a yeast genomic library in phage lambda gt11. Four different clones from the library all contain different overlapping regions of Pdi1p.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Cell membrane preparation (Saccharomyces cerevisiae)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 10mM Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)>90% pure based on SDS-PAGE analysis.
  • ポリ/モノモノクローナル
  • クローン名38H8
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/5000. (gives an ER staining pattern on yeast cells).
WB Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 58.2 kDa). Suggested working dilution of 1/5000 (see PMID: 17051412).

ターゲット情報

  • 関連性PDI1 is a protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds.
  • 参照データベース
    • 別名
      • MFP1 antibody
      • PDI1 antibody
      • Pdi1p antibody
      • Protein disulfide isomerase antibody
      • Thioredoxin related glycoprotein 1 antibody
      • TRG1 antibody
      see all

    Anti-PDI1 antibody [38H8] (ab4644) 使用論文

    This product has been referenced in:
    • Delic M  et al. Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast. Free Radic Biol Med 52:2000-12 (2012). WB . Read more (PubMed: 22406321) »
    • Damasceno LM  et al. Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Appl Microbiol Biotechnol 74:381-9 (2007). Read more (PubMed: 17051412) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. I can confirm this antibody was screened by immunocytochemistry, selecting for various clones that stained yeast cells in an interesting and robust fashion. This particular antibody stains ER and the nuclear membrane, wh...

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    Thank you for contacting us.

    Regrettably, we will not be able to guarantee the anti PDI antibodies cross reactivity towards P. pastoris species. The sequence analysis of ab30319 shows only 45% homology which is very less for antibody cross rea...

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    Thank you for your email. I apologize for your disappointment. I will try to explain what could have happen. The antibodies ab4644 and ab34772 were dispatched on 4th july and were delivered on 7th July. I am not sure if the person who received t...

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    Unfortunately, we are not aware of any publications that feature the use of this antibody.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"