製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PDI
  • 由来種
    Rabbit
  • 特異性
    PDI runs at 61.2 kD on samples from human placenta. In samples from rat liver, it runs at 59 kD, but 3 other bands may appear due to protein degradation - these run at 58.9 kD, 55.2 kD and 53.8 kD.
  • アプリケーション
    適用あり: ICC, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Cow, Human, African green monkey, Chinese hamster
  • 免疫原

    Full length protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 60 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100.
ICC/IF 1/600. PubMed: 19059915

ターゲット情報

  • 機能
    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • 組織特異性
    Highly expressed in pancreas (at protein level).
  • 配列類似性
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 翻訳後修飾
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • 細胞内局在
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

画像

  • ICC/IF image of ab3672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3672, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • Anti-PDI antibody (ab3672) at 1/1000 dilution + PC12 whole cell lysate at 20 µg

    Secondary
    HRP conjugated swine anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 36 kDa, 48 kDa, 58 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

    See Abreview

  • ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Chinese Hamster Ovary cells stained for PDI using ab3672 at 1/200 dilution ICC/IF. Cy3®-labeled secondary antibody.

  • ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr.  Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C.  A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • ab3672 staining PDI - ER in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    See Abreview

参考文献

This product has been referenced in:
  • Charpak-Amikam Y  et al. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20. Sci Rep 7:8661 (2017). Read more (PubMed: 28819195) »
  • Hernández-Tiedra S  et al. Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. Autophagy 12:2213-2229 (2016). Read more (PubMed: 27635674) »

See all 17 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Specification
HEK293
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (ovary)
Specification
ovary
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HaCaT keratinocyte)
Specification
HaCaT keratinocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% triton X-100
Blocking step
2.5% BSA plus 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cortical oligodendrocytes in vibratome sections)
Specification
Cortical oligodendrocytes in vibratome sections
Fixative
Somogy's. PFA + picric acid PLP also, PFA + lysine + periodate
Permeabilization
Yes - TX100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Dr. Guillermo Estivill-Torrus

Verified customer

投稿 Oct 31 2007

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa S3)
Total protein in input
500 µg
Specification
HeLa S3
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

投稿 Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal tumour (phaeochromocytoma))
Specification
adrenal tumour (phaeochromocytoma)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 10 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (testes)
Specification
testes
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 10 2007

1-10 of 13 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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