製品の概要

  • 製品名
    PDH Immunocapture Kit
    Pyruvate dehydrogenase キット 製品一覧
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Cow, Human
  • 製品の概要

    250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of PDH complex from heart mitochondria.

    Note: The immunocapture protocol for this kit requires Abcam reagent detergent lauryl maltoside (ab109857/MS910).

  • アプリケーション
    適用あり: IPmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.

画像

  • Immunoprecipitation - PDH Immunocapture Kit (ab109802)
    Immunoprecipitation - PDH Immunocapture Kit (ab109802)

    PDH immunoprecipitation using antibody ab109866/MSP01c crosslinked to protein G-agarose beads as product ab109802

プロトコール

参考文献

This product has been referenced in:
  • Stenson BM  et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). Read more (PubMed: 19556420) »
  • Bonnet S  et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11:37-51 (2007). Read more (PubMed: 17222789) »

See all 2 Publications for this product

ab109802 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

6 reviews or Q&A

10
Votes: Highest First

How many tests can be performed with each size kit?  Can you please send me a quote (in GBP) for this kit and for an antibody to detect the E1 alpha subunit?

Thank you for contacting us. 
This kit size refers to the amount of antibody.  For example, the 250 ug kit contains 250 ug of antibody conjugated to 25 uL of beads (settled volume) suspended in a total volume of 400 uL. Thus the concentration of t...

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Inquiry: Hi, I am interested in trying this product with rat samples. Do you have any direct evidence for its efficacy against rat PDH, do you know if it was raised against a sequence that is identical to rat? The product information states that it rea...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH c...

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Hello,
I have been already helped because I sent her a separate email amended to my previous inquiry from half a year ago. I also got another reference number yesterday from mailto:uk.technical@abcam.com which is different from the one you just ga...

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Thank you for your previous email and I apologize that I'm just now getting around to reading it! I'll close any other correspondance so you won't be bothered again about this enquiry.

If you need any further information about these products ...

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Hello again,
I had contacted you with a question concerning your PDH-capture antibody (ab109802 or MSP01) about half a year ago, and got a very good answer, so I was wondering if I could ask one more thing. I realize that it most likely is not pos...

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Thank you for contacting us with your question, and for your patience while I have been in touch with the lab about the specificity of this antibody.
My contact at the lab has responded:
"We did mass spectrometry and immunoblotting using...

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Hello, I'm interested in your PDH capture antibody (MSP01) but I was wondering if you could tell me, whether the antibody captures the whole complex along with the kinases and phosphatases. So not just E1a, E1b, E2, E3, E3BP but also the PDKs (PDK1...

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Thank you for contacting us and for your interest in MSP01 (ab109802). I forwarded your email to the lab scientists at MitoSciences, and I received the following response: We do not know if the kinase and phosphatase are present, but if...

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I have a customer interested in the following product:

# MSP01-500 (ab109802) Pyruvate Dehydrogenase Complex (PDH) Immunocapture kit

Can you give evidence to prove this immunocapture works. She is planning to apply this kit in mouse t...

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Thank you for contacting us.

We haven't tested this kit with mouse samples so we unfortunately cannot provide any details. The kit has been optimized with cow and human samples so these samples are fully guaranteed.

We however recomme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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