Anti-PDGFR beta (phospho Y740) 抗体 [EP2135Y] (ab81301)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2135Y] to PDGFR beta (phospho Y740)
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-PDGFR beta (phospho Y740) antibody [EP2135Y]
PDGFR beta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2135Y] to PDGFR beta (phospho Y740) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human PDGFR beta (phospho Y740). The exact sequence is proprietary.
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ポジティブ・コントロール
- NIH 3T3 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2135Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81301の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 190 kDa (predicted molecular weight: 124 kDa).
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特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 190 kDa (predicted molecular weight: 124 kDa). |
ターゲット情報
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機能
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
関連疾患
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 18596 Mouse
- Entrez Gene: 24629 Rat
- SwissProt: P05622 Mouse
- SwissProt: Q05030 Rat
- Unigene: 4146 Mouse
- Unigene: 98311 Rat
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別名
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
画像
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All lanes : Anti-PDGFR beta (phospho Y740) antibody [EP2135Y] (ab81301) at 1/2000 dilution
Lane 1 : NIH 3T3 un-treated
Lane 2 : NIH 3T3 treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 124 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab81301 は 2 報の論文で使用されています。
- Klement W et al. A pericyte-glia scarring develops at the leaky capillaries in the hippocampus during seizure activity. Epilepsia 60:1399-1411 (2019). PubMed: 31135065
- Arango-Lievano M et al. Topographic Reorganization of Cerebrovascular Mural Cells under Seizure Conditions. Cell Rep 23:1045-1059 (2018). WB, IHC (PFA fixed) ; Mouse . PubMed: 29694884