Thia antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
Immunocytochemistry/ Immunofluorescence - Anti-PDGF Receptor beta (phospho Y1021) antibody (ab62437)This image is courtesy of an anonymous Abreview
ab62437 staining PDGF Receptor beta (phospho Y1021) (green) in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - phalloidin.
Immunohistochemical analysis of paraffin embedded human brain tissue using ab62437 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
Western blot - Anti-PDGF Receptor beta (phospho Y1021) antibody (ab62437)This image is courtesy of an Abreview submitted by Lars Ruether
All lanes : PDGF Receptor beta (phospho Y1021) antibody (ab62437) at 1/100 dilution (in TBST for 12 hours at 4°C) + 100µg whole cell lysate of Human glioblastoma (A172 cells)(unstimulated Lane 1 or stimulated Lanes 2-7)
Secondary: A Biotin-conjugated Goat polyclonal at 1/10000 dilution developed using the ECL technique
Blocking Step: 5% BSA for 90 minutes at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF Receptor beta (phospho Y1021) antibody (ab62437)The image is courtesy of an anonymous Abreview
ab62437 staining the PDGF Receptor beta (phospho Y1021) in epithelial cells in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 20 minutes at room temperature; antigen retrieval was by heat mediation with citric acid (pH6.0). Samples were incubated with primary antibody (1/50 in 0.5% BSA/PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.