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Synthetic phosphopeptide derived from human PDGF Receptor beta around the phosphorylation site of tyrosine 1021 (N-D-YP-I-I).
Our Abpromise guarantee covers the use of ab62437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ab62437 staining PDGF Receptor beta (phospho Y1021) (green) in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - phalloidin.
This image is courtesy of an Abreview submitted by Lars RuetherAll lanes : PDGF Receptor beta (phospho Y1021) antibody (ab62437) at 1/100 dilution (in TBST for 12 hours at 4°C) + 100µg whole cell lysate of Human glioblastoma (A172 cells)(unstimulated Lane 1 or stimulated Lanes 2-7)
ab62437 has not yet been referenced specifically in any publications.