Anti-PDGF Receptor beta 抗体 [Y92] (ab32570)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000. Predicted molecular weight: 124 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100.
IP 1/20.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25077029

ターゲット情報

  • 機能Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • 関連疾患Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

Anti-PDGF Receptor beta antibody [Y92] 画像

  • All lanes : Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/10000 dilution (purified)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 124 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human spleen with purified ab32570 at a working dilution of 1/50. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of NIH/3T3 cells with purified ab32570 at a working dilution of 1 in 100, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab32570 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • ab32570 (purified) at 1/20 immunoprecipitating PDGF receptor beta in NIH/3T3 (Lane 1 and 2). Lane 3 - PBS. For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration: 5% NFDM /TBST.
  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/50000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 124 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 124 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 124 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence analysis of NIH3T3 cells stimulated with PDGF, staining PDGF Receptor beta with unpurified ab32570.

Anti-PDGF Receptor beta antibody [Y92] (ab32570) 使用論文

This product has been referenced in:
  • Wymeersch FJ  et al. Position-dependent plasticity of distinct progenitor types in the primitive streak. Elife 5:e10042 (2016). IHC-Fr ; Mouse . Read more (PubMed: 26780186) »
  • Lorusso B  et al. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells. Biomed Res Int 2015:747864 (2015). ICC/IF ; Human . Read more (PubMed: 26137493) »

See all 35 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step 10% NGS-2% Horse Serum in 0.1% Triton X-100-PBS as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 24°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Brandon Jun

Verified customer

投稿 Jan 19 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Baboon Cell (Placental Villus)
Specification Placental Villus
Permeabilization Yes - Triton X-100 (0.1%)
Fixative 10% Buffered Formalin
Username

Mark Olson

Verified customer

投稿 Dec 31 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Mouse Tissue sections (muscle tissue)
Specification muscle tissue
Permeabilization Yes - 0.5%Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 13 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (primary embryonic epicardial)
Specification primary embryonic epicardial
Permeabilization Yes - 0.5% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Methacarn
Username

Abcam user community

Verified customer

投稿 Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cit (pH6) better than Tris (pH8)
Sample Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Methacarn
Username

Abcam user community

Verified customer

投稿 Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (rat cortex)
Specification rat cortex
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 % Triton X-100 in blocking buffer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Da Liu

Verified customer

投稿 Apr 08 2013

The ICC/IF protocol is given through the link below:
http://www.epitomics.com/pdf/ICC.pdf
The cells stained in ICC/IF on the datasheet were NIH-3T3 cells.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Cell (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Feb 18 2013

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