リコンビナント
RabMAb

Anti-PDGF Receptor beta 抗体 [Y92] (ab32570)

製品の概要

  • 製品名
    Anti-PDGF Receptor beta antibody [Y92]
    PDGF Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y92] to PDGF Receptor beta
  • 由来種
    Rabbit
  • 特異性
    This antibody recognizes human platelet-derived growth factor (PDGF) receptor beta. It does not cross-react with other CSF-1/PDGF receptor family members. Expression levels of the target protein vary with sample type and some optimisation may be required.
  • アプリケーション
    適用あり: IHC-Fr, Flow Cyt, IHC-FoFr, WB, IHC-P, ICC/IF, IP, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PDGF Receptor beta aa 1050 to the C-terminus. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: NIH 3T3 cell lysate. ICC/IF: NIH 3T3 cells. IHC-P: Human prostatic carcinoma tissue. Flow Cyt: NIH 3T3 cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/20.
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 124 kDa.

For samples expressing low levels of PDGFR beta, the amount of lysate loaded may need to be increased to allow detection. 

IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Optimisation of the IHC protocol may be required depending on the sample used.

ICC/IF 1/100.
IP 1/20.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25077029

ターゲット情報

  • 機能
    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • 関連疾患
    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

画像

  • ab32570 staining PDGF in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Showes positive staining on glomerulus and stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). A HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

  • All lanes : Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/10000 dilution (purified)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human spleen with purified ab32570 at a working dilution of 1/50. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of NIH/3T3 cells with purified ab32570 at a working dilution of 1 in 100, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab32570 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Flow cytometry analysis of NIH/3T3 (mouse embryo) cells labeling PDGF Receptor beta (red) with ab32570 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • ab32570 staining PDGF in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Showes Positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). A HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

  • ab32570 (purified) at 1/20 immunoprecipitating PDGF receptor beta in NIH/3T3 (Lane 1 and 2). Lane 3 - PBS. For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration: 5% NFDM /TBST.
  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/50000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGF Receptor beta antibody [Y92] (ab32570) at 1/10000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa (why is the actual band size different from the predicted?)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence analysis of NIH3T3 cells stimulated with PDGF, staining PDGF Receptor beta with unpurified ab32570.

  • ab32570 staining PDGF in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Showes Positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). A HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

  • ab32570 staining PDGF in Rat Kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Showes positive staining on glomerulus and stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). A HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

参考文献

This product has been referenced in:
  • Abou-Antoun TJ  et al. Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance. PLoS One 13:e0189711 (2018). Read more (PubMed: 29298329) »
  • Assis-Nascimento P  et al. EphB3 signaling induces cortical endothelial cell death and disrupts the blood-brain barrier after traumatic brain injury. Cell Death Dis 9:7 (2018). Mouse . Read more (PubMed: 29311672) »

See all 78 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen, liver, intestine and kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Spleen, liver, intestine and kidney
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Username

Victor Bernal

Verified customer

投稿 Mar 19 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung)
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 28 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Prostate)
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Unfixed
Username

Abcam user community

Verified customer

投稿 Dec 29 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
10% NGS-2% Horse Serum in 0.1% Triton X-100-PBS as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 24°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Brandon Jun

Verified customer

投稿 Jan 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Baboon Cell (Placental Villus)
Specification
Placental Villus
Permeabilization
Yes - Triton X-100 (0.1%)
Fixative
10% Buffered Formalin
Username

Mark Olson

Verified customer

投稿 Dec 31 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 4°C
Sample
Mouse Tissue sections (muscle tissue)
Specification
muscle tissue
Permeabilization
Yes - 0.5%Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 13 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (primary embryonic epicardial)
Specification
primary embryonic epicardial
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methacarn
Username

Abcam user community

Verified customer

投稿 Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cit (pH6) better than Tris (pH8)
Sample
Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Permeabilization
No
Fixative
Methacarn
Username

Abcam user community

Verified customer

投稿 Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2013

1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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