Anti-PDGF Receptor alpha 抗体, prediluted (ab15501)

製品の概要

  • 製品名Anti-PDGF Receptor alpha antibody, prediluted
    PDGF Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDGF Receptor alpha, prediluted
  • アプリケーション適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) corresponding to C terminal of human PDGFR alpha.

  • ポジティブ・コントロール
    • Human skin and Ovarian Carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 18559345
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. USE NEAT.

ターゲット情報

  • 機能Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 組織特異性Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 関連疾患Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha antibody, prediluted 画像

  • ab15501 staining PDGF Receptor alpha in human ovarian carcinoma by Immunohistochemistry (FFPE-sections).

  • Immunohistochemical analysis of frozen mouse embryonic submandibular gland tissue, staining PDGF Receptor alpha with ab15501.

    Sections were blocked with 1% BSA before incubation with primary antibody. An Alexa Fluor®488-conjugated anti rabbit IgG was used as the secondary antibody.

Anti-PDGF Receptor alpha antibody, prediluted (ab15501) 使用論文

This product has been referenced in:
  • Benedykcinska A  et al. Generation of brain tumours in mice by Cre-mediated recombination of neural progenitors in situ with the tamoxifen metabolite endoxifen. Dis Model Mech 9:211-20 (2016). IHC-P ; Mouse . Read more (PubMed: 26704996) »
  • Yamamoto S  et al. Platelet-derived Growth Factor Receptor Regulates Salivary Gland Morphogenesis via Fibroblast Growth Factor Expression. J Biol Chem 283:23139-23149 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18559345) »

See all 3 Publications for this product

Product Wall

As far as we are aware, there are no published references that specifically feature these two antibodies. However, there are super images on each of the datasheets showing that the antibodies work well in immunohistochemistry and information is provide...

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Thank you for your phone call. Unfortunately there is not an image available showing the skin testing. Both Human skin and Ovarian Carcinoma are recommended as positive controls for ab15501 and there is an image showing the staining with the ovaria...

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I am sorry to hear you are having problems with ab15501. I would like to suggest an antigen retrieval step of boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min as recommended on the d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"